{"id":4202,"date":"2020-03-13T15:57:39","date_gmt":"2020-03-13T15:57:39","guid":{"rendered":"https:\/\/www.innovationnewsnetwork.com\/?p=4202"},"modified":"2020-04-01T13:44:10","modified_gmt":"2020-04-01T12:44:10","slug":"mip2sensors-detecting-and-quantifying-pesticides-in-olive-oil","status":"publish","type":"post","link":"https:\/\/www.innovationnewsnetwork.com\/mip2sensors-detecting-and-quantifying-pesticides-in-olive-oil\/4202\/","title":{"rendered":"MIP2Sensors: detecting and quantifying pesticides in olive oil"},"content":{"rendered":"

Evora University<\/a> present their latest research which encompasses the design and development of new functional selective sorbents for the isolation\/preconcentration of pesticide in olive oil samples.<\/h2>\n

Olive oil (OO) production plays a crucial role within the national economy of Mediterranean countries, including Portugal. To avoid possible losses at harvest or in production, several pesticides are applied to olive groves \u2013 namely organophosphorus and pyrethroids insecticides. To ensure consumer safety, the levels of pesticide residues in OO must be monitored with accurate methodologies. Previously, a tedious and laborious step of sample preparation was mandatory in order to pre-concentrate\/isolate the target pesticide. Standard methods for this quantification are based on the chromatographic techniques \u2018High Performance Liquid Chromatography\u2019 (HPLC) and \u2018Gas Chromatography\u2019(GC), coupled to mass spectrometry (MS). The development of more sensitive, selective and automated techniques for pesticide testing in OO is highly warranted \u2013 our research group has been following a path in order to achieve this challenging goal.<\/p>\n

\"\"
Fig. 1 Analytical workflow for pesticide trace analysis in OO encompassing the use of MIP-based sorbents in the sample preparation step; templates: dimethoate and deltamethrin<\/figcaption><\/figure>\n

Our works focus<\/h3>\n

Aiming to circumvent the lack of selectivity of the most common commercial sorbents for solid phase extraction (SPE) used in the sample preparation step, our research group developed a new and selective approach based on the use of molecularly imprinted polymers (MIP) as SPE, and implemented an analytical workflow for the detection and quantification of pesticide residues in OO (see Fig. 1). MIPs are produced by molecular imprinting technology (MIT), which is a template-direct technique which allows for the design of polymeric materials with well-defined artificial generated recognition sites that are specifically engineered for a target analyte or class of analytes.<\/p>\n

As depicted in Fig.1, the imprinting process (which is the first step) uses a template molecule and a functional monomer for co-polymerisation in the presence of a cross-linking agent, leading to three- dimensional polymer formation. After the removal of the imprinted molecules, the resulting MIP will contain specific cavities or imprints that are sterically and chemically complementary to the template molecule. Theoretically, if the system was designed properly, enabling the polymer to selectively \u201crebind\u201d the imprint molecule and its analogues from a mixture. Since MIPs rely on affinity interactions and offer a higher degree of sample clean-up than that achieved using conventional type SPE sorbents, the selectivity is significantly improved. Subsequently, the interfering background can be reduced and much lower detection limits relative to other less selective sample preparation techniques are achieved using an MISPE approach (referred to as step 2).<\/p>\n

\"\"
Fig. 2 \u201cSmart\u201d MIPs with dual responsive blocks<\/figcaption><\/figure>\n

A step forward in MISPE technology \u2013 the development of \u2018smart\u2019 MIP-based sorbents<\/h3>\n

Aiming to achieve an easier separation from the matrix and a controllable uptake\/release of pesticide residues, advanced functional MIP-based materials have been designed and produced in our lab and further introduced in the sample preparation step during the analysis of pesticide residues in OO. Since these materials are \u201cstimuli- responsive\u201d (known as \u201csmart-MIPs\u201d), a \u201con-off\u201d switchable tool for OO sample preparation that merges molecularly imprinting technology with stimuli-responsive blocks- namely magnetic and photonic stimulus, has also been successfully implemented with both high selectivity and recovery rates (see Fig. 2).<\/p>\n

Moreover, the development of \u201csmart\u201d- MIPs with a dual character- photo- and magnetic-, enables fast and simple separation from the matrix, while the photoresponsiveness property offered simple template elution by using UV\u2013Vis irradiation, proving their suitability for trace pesticide analysis in a complex sample, as OO with low detection limits.<\/p>\n

Exploring a way to bring automation into pesticide trace analysis<\/h3>\n

Our research group is studying the possibility of developing a selective, reliable and robust sensor technology named as MIP2Sensors, encompassing MIPs as recognition elements with an additional \u201clight-up\u201d signaling of the target pesticide presence. Within the development of this sensing tool, it is expected enhancements in selectivity, stability, robustness, portability and will also be easy handling. Thus, an \u201con-site\u201d monitoring of pesticide residues levels in a highly complex matrix will be achieved with remarkable advantages.<\/p>\n

Raquel Garcia<\/strong>
\nMaria Jo\u00e3o Cabrita<\/strong>
\nMED \u2013 Mediterranean Institute for Agriculture, Environment and Development<\/strong>
\nDepartamento de Fitotecnia <\/strong>
\nEscola de Ci\u00eancias e Tecnologia Universidade de \u00c9vora<\/strong>
\n+351 266 760800<\/strong>
\nraquelg@uevora.pt<\/a><\/strong>
\npesticides in olive oilmjbc@uevora.pt<\/a><\/strong><\/p>\n

Please note, this article will also appear in the first edition of our new quarterly publication. Subscribe to our updates for free <\/strong><\/em>here<\/strong><\/em><\/a>.<\/strong><\/em><\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

Evora University present their latest research which encompasses the design and development of new functional selective sorbents for the isolation\/preconcentration of pesticide in olive oil samples. Olive oil (OO) production plays a crucial role within the national economy of Mediterranean countries, including Portugal. To avoid possible losses at harvest or in production, several pesticides are […]<\/p>\n","protected":false},"author":1,"featured_media":4205,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[24433,785],"tags":[529,589,10836],"acf":[],"yoast_head":"\nMIP2Sensors: detecting and quantifying pesticides in olive oil<\/title>\n<meta name=\"description\" content=\"Evora University present their latest research which encompasses the design and development of new functional selective sorbents.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" 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