\u00a9 shutterstock<\/figcaption><\/figure>\nDr Freddie Kalaitzis from the University of Oxford\u2019s Department of Computer Science led the application of Machine Learning methods to microhabitat data. He said: \u201cThis work demonstrates an AI-guided protocol for searching for life on a Mars-like terrestrial analogue on Earth. This protocol is the first of its kind trained on actual field data, and its application can, in principle, generalise to other extreme life-harbouring environments. Our next steps will be to test this method further on Earth with the aim that it will eventually aid our exploration for biosignatures elsewhere in the solar system, such as Mars, Titan, and Europa.\u201d<\/span>\u00a0<\/span><\/p>\nOn Earth, one of the most similar analogues to Mars is the Pajonales, a four-million-year-old lakebed. This area is considered to be inhospitable to most forms of life. Comparable to the evaporitic basins of Mars, the high altitude (3,541 m) basin experiences exceptionally strong levels of ultraviolet radiation, hypersalinity, and low temperatures.<\/span>\u00a0<\/span><\/p>\nWater availability is likely to be the key factor determining the position of biological hotspots<\/span>\u00a0<\/span><\/h3>\nThe researchers collected over 7,700 images and 1,150 samples and tested for the presence of photosynthetic microbes living within the salt domes, rocks, and alabaster crystals that make up the basin\u2019s surface. Here, biosignature markers, such as carotenoid and chlorophyll pigments, could be seen as orange-pink and green layers respectively.<\/span>\u00a0<\/span><\/p>\nGround sampling data and 3D topographical mapping were combined with the drone images to classify regions into four macrohabitats (metre to kilometre scales) and six microhabitats (centimetre scale). The team found that the microbial organisms across the study site were clustered in distinct regions, despite the Pajonales having a near-uniform mineral composition.\u00a0<\/span>\u00a0<\/span><\/p>\nFollow-up experiments showed that rather than environmental variables, like nutrient or light availability, determining the position of, biological hotspots water availability is the most likely factor.\u00a0<\/span>\u00a0<\/span><\/p>\n