The key to the activity of B12-dependent radical SAM enzymes is a simple yet powerful mechanism for triggering the catalytic reaction. The difficulty in getting the enzyme to simultaneously accommodate all the actors involved in the reaction has meant that little structural information had been available. This results in little to no available information that could help to explain how the reaction works.<\/p>\n

To remedy this, the research team combined crystallographic results with biochemical and biophysical data to explain how B12-dependent radical SAM proteins regulate their activity, down to atomic-level details. The Mmp10 enzymatic mechanism was imaged with all actors of the reaction present.<\/p>\n

The results of this research have implications for the development of biotechnologies that would control key enzymatic events, particularly those implicated in methane emission.<\/p>\n

\u201cA total of 137 proteins were screened at a leading synchrotron facility in France to get a glimpse of these rare events, which are so difficult to catch. This research also opens the door to biotech developments,\u201d concluded Co-author and Professor, Leo Chavas, of Nagoya University.<\/p>\n","protected":false},"excerpt":{"rendered":"

Researchers use biophysical and biochemical techniques to analyse the archaea microorganism, that produces methane emissions during anaerobic environments, to attempt to reduce future natural methane emissions. While methane accounts for about 16% of greenhouse gases in the atmosphere – which also include carbon dioxide, nitrous oxide, water vapor \u2013 it is more than 25 times […]<\/p>\n","protected":false},"author":19,"featured_media":19684,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[24429],"tags":[3478,763,689,789],"acf":[],"yoast_head":"\n